Comparison of Bacteria ERIC-PCR Fingerprints of Index Fingers and Contactants / 法医学杂志

Objective To explore the bacteria relevance between index fingers and contactant' surfaces (mobile phone touch screen and desktop of personal office table). Methods Bacteria were collected from the index fingers, mobile phone touch screen and desktop of personal office table of 10 volunteers. Enterobacterial repetitive intergenic consensus(ERIC)-PCR fingerprint was established by PCR amplifi-cation technique of metagenome. Results There were 7 volunteers' ERIC-PCR fingerprints of index fin-gers matched that took from the mobile phone touch screens, and different from each other. There were 3 volunteers' ERIC-PCR fingerprints of index fingers matched that took from desk top of personal office table, and other 7 volunteers' ERIC-PCR fingerprints did not match perfectly with that took from desk top of personal office table,but had at least one similar band for both. Conclusion The bacteria on index finger shows individual specificity, which on mobile phone touching screen and personal desktop may be a new biological sample of forensic identification.

Retrospective analysis of 134 fatal cases with mental disorders during 2013 to 2016 / 复旦学报（医学版）

Objective To analyze the forensic characteristics of deaths in patients with mentally disordered and to provide instructions for forensic evaluation and clinical care.Methods All fatal cases were collected from Hongkou Branch of Shanghai Public Security Bureau from 2013 to 2016.The causes of death,manners of death and their relationship with other factors were statistically analyzed based on systemic case investigation.Results There were a total of 134 fatal cases with mental disorders.The male female ratio was 1 ∶ 1.23 and the mean age at death was (54.9 ± 17.6) years old.In all patients 89 cases (66.4％) had a clinical course of mental illness over 3 years,and 106 cases (79.1％) had a regular prescription of medications prior to deaths.Depression was the most common entity (94 cases),followed by schizophrenia (35 cases) and anxiety (5 cases).According to the manner of death,117 cases (87.3％) were suicidal,16 cases (11.9％) were sudden unexpected deaths,and 1case (0.7％) was accidental death.Fall was the most frequent manner in the suicidal cases (79 cases,58.9 ％).Compared with suicides,cases that died suddenly and unexpectedly had a significantly longer course of mental disorder and medication use,and were more likely occurred inside their residences.Conclusions The psychological status of patients with mentally disordered should be intensively monitored,and their physical health should also be taken care of.

Analysis of 54 mental disability reappraisal cases due to brain damage / 法医学杂志

OBJECTIVE@#To investigate the different kinds of controversial cases of mental disability after brain damage, to analysis the problems in the first appraisal, and to explore solutions of the problems.@*METHODS@#The reappraisals of mental disorders after traumatic brain damage were collected from 2007-2011 in Shanghai forensic center, and the first appraisal and reappraisal cases were analyzed and compared.@*RESULTS@#The changes of conclusion in reappraisal cases showed the following major reasons: inappropriate appraisal time, not comprehensive and object investigation of mental state of patients in first appraisal, misunderstanding the standards, etc.@*CONCLUSION@#The quality improvement of appraisal should adopt the following measures: regulating the practice, improvement of the professional skills of experts, choosing appropriate appraisal time, improvement of appraisal standards, etc.

Vascularized pedicle iliac crest for the repair of bone and soft tissue defect of lower extremity / 中华外科杂志

<p><b>OBJECTIVE</b>To study the role of transplantation of the vascularized pedicle iliac crest for the repair of bone and soft tissue defect of lower extremity.</p><p><b>METHODS</b>The vascularized pedicle iliac crest was designed for the repair of bone and soft tissue defect of lower extremity according to anatomic feature of leg and foot. Skin graft was used for coverage of the iliac flap.</p><p><b>RESULTS</b>Skin survival could demonstrate the survival of the vascularized pedicle iliac crest indirectly one week postoperatively. Skin survived completely in 4 cases and partly in 3 cases. Callus was seen at the transplantation site one month postoperatively, and K-wires were removed 4 months later in the cases of metatarsal defect. The external fixators were removed in the cases of tibia defect 6 to 8 months postoperatively. The functions of lower extremities were restored in 2 to 4 months. The bone and soft tissue defects were repaired, and ultimate function and cosmetic effects were satisfied after the mean follow up of 10 months (ranged from 6 to 15 months).</p><p><b>CONCLUSION</b>Transplantation of the vascularized pedicle iliac crest is an ideal method for the repair of bone and soft tissue defect of lower extremity. The operation can be performed in one stage. The functions and cosmetic effects are better than the traditional methods.</p>

Construction of eucaryotic expression plasmid carrying the BMP7 gene and expression in mesenchymal stem cells / 中华外科杂志

<p><b>OBJECTIVES</b>To construct an eucaryotic expression plasmid carrying the BMP7 gene and express in MSCs.</p><p><b>METHODS</b>The BMP7 gene was cloned into the eucaryotic expression vector pcDNA3.1. At the same time, mesenchymal stem cells (MSCs) were isolated and cultured in vitro. The plasmid carrying the BMP7 gene was transfected into MSCs.</p><p><b>RESULTS</b>PCR and digesting demonstrated that the eucaryotic expression plasmid -pcDNA-BMP7 was obtained. RT-PCR and immunohistochemical methods showed that the BMP7 gene was expressed in MSCs.</p><p><b>CONCLUSION</b>Construction of an eucaryotic expression plasmid carrying BMP7 gene and expression in MSCs provide a sound basis for gene therapy using the BMP7 gene and the ideal seeds for tissue engineering.</p>

Experimental study of the isolation, culture and in chondrogenic differentiation of human bone mesenchymal stem cell / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the isolation of human bone marrow mesenchymal stem cells (MSCs) and in vitro differentiation into chondrocytes as potential seed cell for condyle cartilage tissue engineering.</p><p><b>METHODS</b>Human MSCs were isolated by percoll solution from normal human bone marrow sample and cultured in flasks. Specific cell surface markers were identified by flow-cytometry. After the cells were treated with inductive medium containing insulin, transferrin, pyruvate, dexathemesone and TGF-beta for 7 - 14 days, microscopic, histological and immuno-histo-chemical studies were performed for chondrogenic phenotype identification.</p><p><b>RESULTS</b>Primary cultures of human MSCs express CD29 and CD44 positively and meanly, but CD34, CD45 and HLA-DR negatively. After 14 days of induction, the cells were positively stained by safranin O. Immunohistochemical analysis proved strong type II collagen expression.</p><p><b>CONCLUSIONS</b>Percoll helps to generate a better isolation of MSCs from human bone marrow aspirates with a purity more above 95%. The isolated MSCs can be expanded and induced in vitro to differentiate into chondrocytes by inductive medium.</p>